RP-HPLC and HPTLC method development and validation for estimation of dolutegravir in bulk and tablet dosage form
By: Ghante, M. R
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Contributor(s): Sawant, S. D.
Publisher: Mumbai Indian Drug Manufacture's Association - IDMA 2019Edition: Vol56(5), May.Description: 30-38p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian drugsSummary: Dolutegravir (DTG) is a second-generation integrase strand transfer inhibitor (INSTI), antiretroviral drug. RP-HPLC and HPTLC methods have been developed and validated for DTG in bulk and tablet dosage form. In HPLC method, analysis was carried out on C-18 column using a mixture of acetonitrile: phosphate buffer (pH 7.0) in the ratio of (90:10 V/V) as mobile phase at the flow rate 1 mL/min at 261 nm. The method was found to be linear in the concentration range 10-70 μg/mL. Peak for DTG was observed at 2.8 ± 0.1 minutes. For HPTLC method proper resolution was achieved with toluene: methanol: formic acid 7:3:0.1(V/V/V) as the mobile phase at 334 nm. HPTLC method was found to be linear in concentration range of 200-600 ng/band. Rf value of DTG was found to be 0.496 ± 0.0055. Developed methods were validated as per ICH guidelines Q2(R1) and were found to be accurate, precise, simple and robust.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2020651 |
Dolutegravir (DTG) is a second-generation integrase strand transfer inhibitor (INSTI), antiretroviral drug. RP-HPLC and HPTLC methods have been developed and validated for DTG in bulk and tablet dosage form. In HPLC method, analysis was carried out on C-18 column using a mixture of acetonitrile: phosphate buffer (pH 7.0) in the ratio of (90:10 V/V) as mobile phase at the flow rate 1 mL/min at 261 nm. The method was found to be linear in the concentration range 10-70 μg/mL. Peak for DTG was observed at 2.8 ± 0.1 minutes. For HPTLC method proper resolution was achieved with toluene: methanol: formic acid 7:3:0.1(V/V/V) as the mobile phase at 334 nm. HPTLC method was found to be linear in concentration range of 200-600 ng/band. Rf value of DTG was found to be 0.496 ± 0.0055. Developed methods were validated as per ICH guidelines Q2(R1) and were found to be accurate, precise, simple and robust.
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